Title
A simple nuclear contrast staining method for microCT‐based 3D histology using lead(II) acetate
Author
Abstract
X-ray microtomography (microCT) enables histological-scale 3D imaging of many types of biological samples, but it has yet to rival traditional histology for differentiation of tissue types and cell components. This report presents prima facie results indicating that a simple lead(II) acetate staining solution can impart preferential X-ray contrast to cell nuclei. While not strictly selective for nuclei, the staining reflects local cell-density differences. It can be applied in a single overnight treatment and does not require hematoxylin staining or drying of the sample. The stain is removable with EDTA, and it may enhance early calcifications. A basic protocol is given as a guide for further testing and optimization.
Keywords
3D histologyhematoxylinimaginglead(II) acetatelimb developmentmorphologystainingX-ray microtomography
Object type
Language
English [eng]
Persistent identifier
Appeared in
Title
Journal of Anatomy
Volume
238
Issue
4
ISSN
0021-8782
Issued
2020
From page
1036
To page
1041
Publication
Wiley
Version type
Date issued
2020
Access rights
Rights statement
© 2020 The Authors
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