Titel
Functional characterization of the human tRNA methyltransferases TRMT10A and TRMT10B
Autor*in
Ursula Toth
Center for Anatomy & Cell Biology, Medical University of Vienna
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Abstract
The TRM10 family of methyltransferases is responsible for the N1-methylation of purines at position 9 of tRNAs in Archaea and Eukarya. The human genome encodes three TRM10-type enzymes, of which only the mitochondrial TRMT10C was previously characterized in detail, whereas the functional significance of the two presumably nuclear enzymes TRMT10A and TRMT10B remained unexplained. Here we show that TRMT10A is m1G9-specific and methylates a subset of nuclear-encoded tRNAs, whilst TRMT10B is the first m1A9-specific tRNA methyltransferase found in eukaryotes and is responsible for the modification of a single nuclear-encoded tRNA. Furthermore, we show that the lack of G9 methylation causes a decrease in the steady-state levels of the initiator tRNAiMet-CAT and an alteration in its further post-transcriptional modification. Our work finally clarifies the function of TRMT10A and TRMT10B in vivo and provides evidence that the loss of TRMT10A affects the pool of cytosolic tRNAs required for protein synthesis.
Stichwort
Genetics
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
Nucleic Acids Research
Band
48
Ausgabe
11
ISSN
0305-1048
Erscheinungsdatum
2020
Seitenanfang
6157
Seitenende
6169
Publication
Oxford University Press (OUP)
Erscheinungsdatum
2020
Zugänglichkeit
Rechte
Rechteangabe
© The Author(s) 2020
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