Title
Evaluation of Sustainable Recycled Products to Increase the Production of Nutraceutical and Antibacterial Molecules in Basil Plants by a Combined Metabolomic Approach
Author
Mariavittoria Verrillo
Dipartimento di Agraria, Università di Napoli Federico II
... show all
Abstract
Background: An important goal of modern medicine is the development of products deriving from natural sources to improve environmental sustainability. In this study, humic substances (HS) and compost teas (CTs) extracted from artichoke (ART) and coffee grounds (COF) as recycled biomasses were employed on Ocimum basilicum plants to optimize the yield of specific metabolites with nutraceutical and antibacterial features by applying sustainable strategies. Methods: The molecular characteristics of compost derivates were elucidated by Nuclear Magnetic Resonance spectroscopy to investigate the structure–activity relationship between organic extracts and their bioactive potential. Additionally, combined untargeted and targeted metabolomics workflows were applied to plants treated with different concentrations of compost extracts. Results: The substances HS-ART and CT-COF improved both antioxidant activity (TEAC values between 39 and 55 μmol g−1) and the antimicrobial efficacy (MIC value between 3.7 and 1.3 μg mL−1) of basil metabolites. The metabolomic approach identified about 149 metabolites related to the applied treatments. Targeted metabolite quantification further highlighted the eliciting effect of HS-ART and CT-COF on the synthesis of aromatic amino acids and phenolic compounds for nutraceutical application. Conclusions: The combination of molecular characterization, biological assays, and an advanced metabolomic approach, provided innovative insight into the valorization of recycled biomass to increase the availability of natural compounds employed in the medical field.
Keywords
recycling biomassesmetabolomicsphenolic compoundsantioxidant capacityantibacterial properties
Object type
Language
English [eng]
Persistent identifier
https://phaidra.univie.ac.at/o:1639221
Appeared in
Title
Plants
Volume
12
Issue
3
ISSN
2223-7747
Issued
2023
Publisher
MDPI AG
Date issued
2023
Access rights
Rights statement
© 2023 by the authors
University of Vienna | Universitätsring 1 | 1010 Vienna | T +43-1-4277-0