Title
The single-grain method: adding TEM to the equation
Author
Abstract
An advanced protocol to prepare single extant and fossil pollen grains for transmission electron microscopy (TEM) analysis allows for the fast recovery of data on the ultrastructure of pollen/spores. The protocol is easy to apply and less time consuming than previous methods. The ‘loss’ of pollen grains and pollen that is ‘difficult to locate’ within the embedding material is avoided, and each single pollen grain can be prepared successfully for TEM analysis. This preparation method is meant as an addition to the single-grain method using combined light and scanning electron microscopy to investigate dispersed fossil pollen grains developed by Dr Reinhard Zetter in the late 1980s.
Keywords
fossil pollenlight microscopypollen morphologypollen ultrastructurescanning electron microscopytransmission electron microscopy
Object type
Language
English [eng]
Persistent identifier
phaidra.univie.ac.at/o:2092861
Appeared in
Title
Grana
Volume
59
Issue
1
ISSN
0017-3134
Issued
2019
From page
44
To page
57
Publication
Informa UK Limited
Version type
Date issued
2019
Access rights
Rights
Rights statement
© 2019 The Author(s)
University of Vienna | Universitätsring 1 | 1010 Vienna | T
+43-1-4277-0