Terrestrial adaptation of green algae Klebsormidium and Zygnema (Charophyta) involves diversity in photosynthetic traits but not in CO2 acquisition

Main conclusion The basal streptophyte Klebsormidium and the advanced Zygnema show adaptation to terrestrialization. Differences are found in photoprotection and resistance to short-term light changes, but not in CO 2 acquisition. Streptophyte green algae colonized land about 450–500 million years ago giving origin to terrestrial plants. We aim to understand how their physiological adaptations are linked to the ecological conditions (light, water and CO2) characterizing modern terrestrial habitats. A new Klebsormidium isolate from a strongly acidic environment of a former copper mine (Schwarzwand, Austria) is investigated, in comparison to Klebsormidium cf. flaccidum and Zygnema sp. We show that these genera possess different photosynthetic traits and water requirements. Particularly, the Klebsormidium species displayed a higher photoprotection capacity, concluded from non-photochemical quenching (NPQ) and higher tolerance to high light intensity than Zygnema. However, Klebsormidium suffered from photoinhibition when the light intensity in the environment increased rapidly, indicating that NPQ is involved in photoprotection against strong and stable irradiance. Klebsormidium was also highly resistant to cellular water loss (dehydration) under low light. On the other hand, exposure to relatively high light intensity during dehydration caused a harmful over-reduction of the electron transport chain, leading to PSII damages and impairing the ability to recover after rehydration. Thus, we suggest that dehydration is a selective force shaping the adaptation of this species towards low light. Contrary to the photosynthetic characteristics, the inorganic carbon (Ci) acquisition was equivalent between Klebsormidium and Zygnema. Despite their different habitats and restriction to hydro-terrestrial environment, the three organisms showed similar use of CO2 and HCO3 − as source of Ci for photosynthesis, pointing out a similar adaptation of their CO2-concentrating mechanisms to terrestrial life. Electronic supplementary material The online version of this article (doi:10.1007/s00425-017-2741-5) contains supplementary material, which is available to authorized users.


Introduction
Streptophyte green algae started land colonization about 450-500 million years ago (MYA) and this was an important step for the evolution of terrestrial plants (Becker and Marin 2009;Becker 2013). With the transition to land, ancestors of these organisms had to face new environmental conditions, including exposure to higher solar irradiance compared to the water environment, lower water accessibility and higher pCO 2 than in the extant atmosphere (Becker and Marin 2009;Alboresi et al. 2010;Raven and Colmer 2016). Among streptophytes, Klebsormidium (Klebsormidiophyceae) and Zygnema (Zygnematophyceae) first appeared *500-700 MYA (Leliaert et al. 2011;Becker 2013) and they are classified as belonging to the basal and advanced groups of the streptophyte lineage, respectively (de Vries et al. 2016). The Klebsormidium genome has revealed that this organism acquired many genes specific for a plant terrestrial life (Hori et al. 2014;de Vries et al. 2017). Currently, Zygnema, as belonging to the order of Zygnematales, is among the closest algal relatives of land plants (Timme et al. 2012).
The pioneering behaviour of streptophyte green algae during land colonization is still present in modern habitats where they are abundant in freshwater, hydro-terrestrial habitats (Zygnema; Holzinger and Pichrtová 2016) and biological soil crusts (Klebsormidium; Holzinger and Karsten 2013; worldwide, and where they contribute to important ecological roles as primary production, carbon and nitrogen biogeochemical cycles, and soil stabilization (Elbert et al. 2012). Occurrence in these environments expose cells to various and extreme environmental conditions including long exposure to high light intensities and cellular water loss i.e., dehydration . Under such conditions, photosynthetic resistance against intense light involves the presence of photoprotective mechanisms e.g., energy dissipation as heat (non-photochemical quenching, NPQ; Alboresi et al. 2008;Gerotto et al. 2011;Goss and Lepetit 2015) and/or activation of a nonradiative electron recombination route to reduce ROS production (Ohad et al. 2010;Treves et al. 2016). Klebsormidium and Zygnema have been shown to have different NPQ kinetics that might confer a different sensitivity of their photosynthetic apparatus to high light environments (Gerotto and Morosinotto 2013).
As well as for light, not less important is photosynthetic resistance during dehydration (Heber 2008). In the case of Klebsormidium, transcriptomic analysis showed that dehydration caused up-regulation of genes involved in photosynthesis, showing that the photosynthetic apparatus is prone to acclimation under this condition . However, in nature, dehydration may occur rapidly, without giving time for the transcripts to be translated into proteins and to the cell, the chance to acclimate (Cruz de Carvalho et al. 2014). Moreover, due to the variability of light climate (e.g., daily light changes), frequently dehydration occurs under elevated irradiances which might pose a danger for photosynthesis (Gray et al. 2007; Raanan et al. 2016a). Consequently, high desiccation tolerant species must possess constitutively expressed mechanisms to quickly protect the photosynthetic apparatus and allowing recovery when water is taken up again (Gray et al. 2007;Yamakawa et al. 2012; Bar-Eyal et al. 2015). It is known that Klebsormidium is capable to inactivate photosynthesis during dehydration in low light (40 lmol photons m -2 s -1 ) and recover after rehydration Karsten et al. 2016). However, to better understand its physiological requirements and distribution in the environment, we need to understand the response of its photosynthetic apparatus to dehydration when exposed to different light intensities.
Upon land colonization, besides high light and dehydration tolerance, adaptations for resources acquisition such as inorganic carbon (C i ) are essential to guarantee the species occurrence in a certain environment. C i acquisition has been extensively studied in aquatic algae and many of them are known to possess CO 2 -concentrating mechanisms (CCMs) i.e., structural and functional components whose role is to furnish the cell with CO 2 for photosynthesis (Giordano et al. 2005;Reinfelder 2011). Different CCMs are observed among different species, in relation to the form of C i uptaken (CO 2 vs HCO 3 -) and presence or absence of pyrenoids (Ratti et al. 2007;Brading et al. 2013;Stojkovic et al. 2013). In several streptophyte green algae, including Klebsormidium and Zygnema, functional CCMs can be inferred from the presence of pyrenoids (Meyer et al. 2008;Mikhailyuk et al. 2015), an organelle which, although with some exceptions, is often associated with intracellular C i accumulation and CCM (Smith and Griffiths 1996;Maberly et al. 2009;Villarreal and Renner 2012;Raven et al. 2017). In the case of Zygnema, active CCM are also indicated by low CO 2 compensation point (Birmingham and Colman 1979). In relation to the preferred C i forms, the evolutionary adaptation of these organisms to terrestrial conditions may have favoured their predilection for CO 2 acquisition rather than HCO 3 -. Yet, different preferences for CO 2 or HCO 3 may be expected in relation to single species adaptation to a particular habitat (Lachmann et al. 2016). For instance, in species such as Zygnema with higher restriction to moist environments Lajos et al. 2016), where both CO 2 and HCO 3 are present, lower dependence on CO 2 can be expected. Contrary, for species with higher adaptation to soil or dry conditions as Klebsormidium, CO 2 could represent the preferred source of C i for photosynthesis. However, to our knowledge, the mechanisms whereby these terrestrial streptophyte green algae attain C i have not been investigated so far.
The aim of the present study is to perform a comparison of physiological traits in the genera Klebsormidium and Zygnema in relation to ecological parameters (light, water and C i ) which characterize terrestrial habitats. For our experiments, we compared a new Klebsormidium isolate from an acidic environment with K. cf. flaccidum from a soil crust and a Zygnema sp. isolated from a sandy river shore. The latter two isolates have been characterized by means of phylogeny, structure and ultrastructure as well as some physiological aspects before Mikhailyuk et al. 2015;. These previous observations gave a solid basis for the present study; however, a direct comparison of these two genera in culture medium at the same culture age and with the same methods was not carried out before. We analysed their ability to employ photoprotective mechanisms and how they are linked to terrestrial light conditions. We also characterized the changes of the photosynthetic apparatus during a dehydration/rehydration cycle under different light regimes, aiming to define the role of dehydration in shaping species-specific photosynthesis. Finally, we assessed the presence of CCMs and tested if the acquisition of different C i forms is related to the occurrence of these genera in different habitats.

Species morphology and isolation
We used a Zygnema sp. (Culture collection of Algae Göttingen, SAG 2419, isolated from a sandy river shore,  and two Klebsormidium isolates. These included: (1) a new Klebsormidium isolate with long, tangled filaments, collected from the acidic (pH 4.3) environment of a former mining site termed Schwarzwand (47°9 0 36.84 00 N, 13°13 0 13.28 00 E) (Großarl Valley in Salzburg, Austria, Adlassnig et al. 2013); and (2) the previously described K. flaccidum KUE1 (alpine biological soil crust, Tyrolean Alps, Austria) and grouping into the B-Clade according to ITS-phylogeny ). The latter species name was also modified into K. cf. flaccidum by Mikhailyuk et al. (2015) according to the observation that the filaments had a stronger tendency to disintegrate.

Culture conditions
The two Klebsormidium isolates were grown in modified Bolds Basal Medium (MBBM) and Zygnema in standard BBM culture media, respectively, and buffered at pH 7.5 using 40 mmol L -1 Hepes. Cultures were incubated in a growth chamber with a temperature cycle of 20-15°C of 16:8 h, and exposed to an incident photon flux density of 50-70 lmol photons m -2 s -1 . All species were maintained in batch growth, using 200 mL Erlenmeyer flasks filled with a maximal culture volume of 100 mL. Cultures were refreshed with culture medium regularly (every 2 weeks) to maintain filaments concentration low and to avoid nutrients depletion in the medium. To test a possible/particular adaptation of Klebsormidium isolated from Schwarzwand to low pH environments, results from control experimental condition (pH 7.5) were compared to analyses performed on filaments transferred for 24 h into a MBBM pH 4.1 (buffered with 5 mM citric acid/Na-citrate, Gerloff-Elias et al. 2005). Prolonged (several days) exposure of filaments to low pH caused the culture medium to get 'turbid' which we considered unsuitable for further physiological experiments.

Phylogenetic analyses
The new Klebsormidium strain from Schwarzwand was characterized by rbcL (large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase) marker, which is the most used molecular marker for this streptophytic green algae. The DNA from the strain was isolated according to the protocol of Ryšánek et al. (2015). The sequences of the rbcL gene were obtained using polymerase chain reaction (PCR) amplification with a Touchgene Gradient cycler (Techne, Cambridge, UK). The rbcL gene was amplified using the forward primer KF590 150 (5 0 -GAT GAA AAC GTA AAC TCT CAG C-3 0 ) and the reverse primer rbcL-KR2 (5 0 -GGT TGC CTT CGC GAG CTA-3 0 ) (Š kaloud and Rindi 2013). Each 20 lL reaction solution for PCR was conducted as described by Ryšánek et al. (2015). The PCR protocol followed that of Š kaloud and Rindi (2013). Sequencing reads were assembled and edited using SeqAssem software (Hepperle 2004). Newly obtained Klebsormidium rbcL sequence and the sequences available in the GenBank database were used to produce an alignment for phylogenetic analyses. The final alignment was constructed by ClustalW (Thompson et al. 1994) with MEGA v6.06 (Tamura et al. 2011). The aligned data set was analysed using Bayesian analysis (BI) with MrBayes v3.1.2 (Huelsenbeck and Ronquist 2001), maximum likelihood analysis (ML) with GARLI (Zwickl 2006), and maximum parsimony (MP) analysis with PAUP v4.0b10 (Swofford 2002). The evolutionary model used was the same as in Ryšánek et al. (2015). The BI analysis was performed using the priors set as default in MrBayes; the robustness of the tree topologies was assessed by bootstrapping the data set as described by Š kaloud and Rindi (2013).

Light-and transmission electron microscopy (TEM)
For light microscopy a Zeiss Axiovert 200 M microscope with a 100 9 1.3 NA objective lense was used and transmission electron microscopy was essentially carried out by a classical chemical fixation procedure as previously described (Holzinger et al. 2009). Transmission electron micrographs were captured with a TRS 2k SSCCD camera connected to a Zeiss Libra 120 TEM operated at 80 kV.

Rapid light curves, NPQ and OJIP measurements
The light acclimation status and PSII properties of Klebsormidium and Zygnema were analyzed using a PAM 2500 fluorimeter (Heinz Walz, Effeltrich, Germany). Prior any measurements, samples were dark acclimated for 15 min. Rapid light curves (RLCs), as assessment of the photosynthetic response to rapid increase of light (every 30 s), were obtained by exposing cells to light intensities between 0 and 2014 lmol photons m -2 s -1 . The RLCs were then fitted through the mathematical model of Walsby (1997). Fluorescence induction curves for NPQ estimation were obtained using 20 saturating light pulses (300 ms) upon cells exposed to an actinic light intensity of 1159 lmol photons m -2 s -1 , and followed by a dark recovery time to monitor NPQ relaxation phase. The OJIP (O, origin; J and I, intermediated inflections; P, peak; Stirbet et al. 2014) transients were obtained by a multi turn-over flash generated using the default trigger pattern of PamWin-3 software (Poly300 ms.FTM).

P vs I curves
Rates of photosynthetic O 2 evolution as a function of irradiance (P vs I curve) were used as assessment of photosynthetic response towards relatively slower increase of light intensity. The P vs I curves were measured with a Presens Fibox 3 oxygen optode (Presens, Regensburg, Germany) fixed in a 3-mL thermostatic acrylic chamber (type DW1, Hansatech Instruments, Norfolk, UK) as in Kaplan et al. (2013). Prior each P vs I curve measurement, the cells were dark acclimated for 15 min with the final 5 min of this incubation period used to measure the dark respiration (R d ). Following the dark period, P vs I curves were obtained by exposing the cell suspension to a progressive increase (every 5-10 min) of light intensities between 0 and 1520 lmol photons m -2 s -1 . To avoid the possibility of photorespiration, during the experiments the O 2 concentration in the chamber was maintained between 15 and 60% of air equilibrium (Pierangelini et al. 2014). The P vs I curves and photosynthetic parameters as maximum photosynthetic rate (P max ), light harvesting (a), photoinhibition (b) and onset of light saturated photosynthesis (I k = P max /a) were generated using the model of Walsby (1997). Results were normalized to Chl a. At the end of each P vs I curve, the algal suspension was filtered onto a Whatman GF/C glass microfiber filter, resuspended in 1 mL DMF (with overnight extraction), and the Chl a quantified photometrically using the equations of Porra et al. (1989).

Dehydration and recovery experiment
To study the response of the photosynthetic apparatus when dehydration occurs at both sub-and saturating light intensity for photosynthesis, we performed a dehydration/ rehydration experiment at two light regimes, 25 (LL, low light) and 185 (SL, saturating light) lmol photons m -2 s -1 ; measured with a Solar Light PMA 2132 cosine corrected PAR sensor connected to a Solar Light PMA 2100 radiometer (Solar Light Co., Inc., Philadelphia, PA, USA). Samples were placed under the same light source and the low light treatment was obtained using light screens. The dehydration and rehydration cycle were performed essentially as in Karsten et al. ( , 2016 and in . Filaments of Klebsormidium and Zygnema were collected from the culture, re-suspend in 200 lL of fresh MBBM or BBM media and placed onto a 45-mm membrane filter (mixed cellulose ester, Whatman GmbH, Dassel, Germany). Filters were placed inside the desiccation chamber described in , and filled with 100 mL 3.5 mol L -1 KCl dehydrating solution. Dehydration was allowed to take place for 24 h. After this period, filaments on filters were rewetted with 200 lL of fresh culture medium and the KCl solution in the chamber replaced with 100 mL of tap water. The relative humidity (78.5-94%) during the experiment was recorded using a PCE-MSR145S-TH mini data logger (PCE Instruments, Meschede, Germany). For the assessment of the PSII proprieties, the PAM 2500 probe was placed outside the chamber at the fixed distance of 11 mm from the filter. The effective quantum yield ðYII ¼ ðF 0 m À FÞ=F 0 m Þ was measured on filaments exposed to each respective light intensity. F v /F m and OJIP transients were measured during the dehydration phase on dark acclimated filaments for 15 min. At the end of the rehydration phase of Klebsormidium, F v / F m were measured on filaments collected from the filters and resuspended in fresh MBBM medium.

pH drift and C i acquisition
For the analysis of C i acquisition in Klebsormidium and Zygnema we performed a pH-drift experiment as described by Maberly and Spence (1983) using an artificial assay medium (pH 7.5, alkalinity *1 mEq L -1 ) prepared as in Lachmann et al. (2016). Filaments were harvested from the culture, washed in 20 mL of assay medium, placed in an air tight 25 mL glass vial (obtaining a Chl a concentration between 0.4 and 0.8 lg mL -1 ), and exposed to a maximal incident light intensity of 110 lmol photons m -2 s -1 . The increase of pH of the assay medium was recorded every 30-60 min by quickly opening and introducing the pH probe into the vials. During the measures gas exchange was minimal since the pH probe fitted the vials aperture. At the end of pH drift incubation, the final alkalinity of assay medium was measured by Gran tritration and C i speciation (CO 2 , HCO 3 -, CO 3 -2 ) calculated from the constants of Millero (1979) and the NBS pH scale, using the CO 2-Sys.xls application (Holland et al. 2012). The results were used to estimate the variation of the C i forms during the course of the experiment, to calculate the maximal C i uptake rate normalized to Chl a (extracted as described above), and calculate the quotient of final total C i (C T ) over final alkalinity (C T /Alk, Lachmann et al. 2016).

Statistical analysis
Experiments were performed with at least three biological replicates. We tested the significance of mean differences among the three organisms using one-way ANOVA followed by Bonferroni's multiple comparison test. The variation among means in relation to time was tested using two-way repeated-measures (RM) ANOVA. Comparison between two means was carried out by two-tailed t test. The analyses were performed using the software GraphPad Prism 5, setting the threshold of significance at 95%.

Phylogenetic characterization, light and transmission electron microscopy of the new isolate
While phylogenetic and morphological characterization of the K. cf. flaccidum strain KUE1 Mikhailyuk et al. 2015) and Zygnema sp. (SAG 2419,  were previously available, the new strain isolated from Schwarzwand, Austria, hereafter Klebsormidium sp. (SCHW), was found to group into clade E2 by rbcL analysis (Fig. 1). The cells had an average cell width of 5.5 (±0.5) lm and an average cell length of 9.3 (±1.8) lm, and a parietal chloroplast with a prominent pyrenoid surrounded by numerous starch grains (Fig. 2). The chloroplasts covered at least 2/3 of the inner cell surface. Transmission electron microscopy of the new isolate allowed to further characterize the subcellular organization. The chloroplasts contained prominent pyrenoids (Fig. 3) that were transversed by thylakoid membranes (Fig. 3a). Several pyrenoids were found to be surrounded by starch grains (Fig. 3). The chloroplasts contained several plastoglobules (Fig. 3a, b). The nucleus was found occasionally not in the typical central position, but close to the cross walls, sometimes drastically elongated (Fig. 3a, b).

Maximum quantum yield and Non-photochemical quenching
Similar F v /F m were found among Klebsormidium isolates and Zygnema (P = 0.1746, Table 1). The different NPQ kinetics between Klebsormidium and Zygnema are reported in Fig. 4. Compared to Zygnema, both Klebsormidium isolates showed a higher capacity to perform NPQ. Moreover, differently from Zygnema, the Klebsormidium NPQ was inducible and reaching full activation after a relative long time (*6 min) of exposure to strong actinic light. Klebsormidium sp. (SCHW) showed higher maximal NPQ (P = 0.0107) than K. cf. flaccidum (KUE1). The kinetics of the Klebsormidium NPQ induction reported here are comparable to the results of Gerotto and Morosinotto (2013).

Slow vs rapid increase of light
To understand how the NPQ traits are linked to natural light conditions, we compared the results of P vs I curves with the RLCs. The results of P vs I curves, showing the photosynthetic responses of Klebsormidium isolates and Zygnema to relatively slow increase of light intensity, are reported in Fig. 5a-c and in Table 1. R d (P = 0.3731) and P max (P = 0.9023) were similar among the Klebsormidium isolates and Zygnema. The a was higher for Klebsormidium sp. (SCHW) than in K. cf. flaccidum (KUE1) and Zygnema (P = 0.0155). Reflecting the higher a, the I k was found lower in Klebsormidium sp. (SCHW) (P = 0.0137). Klebsormidium sp. (SCHW) showed negligible b, whereas for K. cf. flaccidum (KUE1) b was null. On the other hand, although statistically weak (P = 0.0574), Zygnema showed higher tendency to photoinhibition. Figure 5d-f show the responses of Klebsormidium isolates and Zygnema to a comparatively faster increase of light intensity (RLCs curves). The most striking observation is that with RLCs all species studied had higher susceptibility to high light intensity (b) than during the P vs I curves.  down to 0.51 (±0.03) (P = 0.0019), suggesting the occurrence of changes/damages at the PSII. The RLCs (Fig. 5d) highlighted a decline of a (P = 0.0134) but rETR max was not altered under the low pH condition (P = 0.5671).

Dehydration and rehydration
As reported by  Zygnema was more sensitive to dehydration than Klebsormidium.
The YII rapidly declined during dehydration and did no recover after 24 h (Fig. S1). Contrary, Klebsormidium sp.
(SCHW) tolerated longer dehydration periods and quickly recovered even after 24 h of being in a dehydrated state (Fig. 6). Due to the dependence of the YII on the light acclimated state (which induces non-photochemical downregulation and reaction centres closure), the absolute differences in YII between the LL and SL treatments can be attributed to the different light regime at which the cells were exposed during the fluorescence measures. Yet, our results showed that the capacity of Klebsormidium to tolerate dehydration and rehydration cycle was influenced by the light exposure. During dehydration, the YII (Fig. 6a) of cells exposed to SL started to drop down to null values earlier (40 min) than for cells under the LL treatment. In SL, the F o measured on dark acclimated cells was higher than in cells under LL (two-way ANOVA RM, Since no changes were observed in the F m (two-way ANOVA RM, P = 0.4683; Fig. 6c), the decrease of F o is considered the cause for the F v /F m decline (two-way ANOVA RM, P = 0.0002; data not shown). The OJIP transients measured on the same dark acclimated cells confirmed these results, showing a higher F o (P = 0.0109), unchanged F m (P = 0.2043) and lower F v /F m (P = 0.0029) in SL than in LL cells ( Fig. 7; Table 2). As well as for dehydration, exposure of cells to SL influenced the recovery of the photosynthetic machinery during rehydration phase (Fig. 6d). While for the cells at LL the YII rapidly returned (within 2 h) to values as high as those measured at dehydration, the YII recovery for cells in SL was slower and stopped at *73% of the YII values measured during dehydration (Fig. 6a). Consistently, after 4 h in rehydrated condition, cells in the SL showed lower F v / F m (0.47 ± 0.03) than at LL which was fully recovered (0.67 ± 0.02) (P = 0.0011), indicating the presence of PSII damages which impaired the cells to recover their photosynthetic capacity.

pH-drift and C i acquisition
The rise of pH during the pH-drift experiment is shown in Fig. 8a. The pH increased rapidly from 7.6 to *9.0 but the following increase up to final and stable values of *9.7 occurred more slowly. The rates of C i uptake as a function of C i (Fig. 8b) did not show any species-specific variation.

Discussion
In this work, we investigated the eco-physiological traits that make Klebsormidium and Zygnema capable to successfully colonize terrestrial habitats. We showed that photosynthetic characteristics are distinct between Klebsormidium and Zygnema (NPQ, photoinhibition), reflecting their preference for different light regimes in natural ecosystems. These streptophyte green algae possess comparable C i acquisition traits, indicating no genera-specific adaptation to habitats but rather an overall C i acquisition adaptation to terrestrial life. The sensitivity of Klebsormidium to light conditions during cellular water loss, emphasises the importance of considering multiple environmental factors when studying the effectiveness of mechanisms involved in protection of the photosynthetic apparatus during dehydration. Regarding the newly isolated Klebsormidium strain from an acidic environment (Schwarzwand, Austria), the physiological comparison showed that rates of R d and P max were similar to K. cf. flaccidum (KUE1). This is interesting, as these two Klebsormidium strains belong to rather distinct clades according to molecular phylogeny. While by means of rbcL phylogeny Klebsormidium sp. (SCHW) was grouping into clade E2, according to the terminology of Rindi et al. (2011), the K. cf. flaccidum strain previously Values in brackets represent standard deviation (n C 3) and asterisks indicate statistically significant differences from Klebsormidium sp. isolated grouped into clade B  or the combined subclades B/C (Mikhailyuk et al. 2015). However, the higher a in Klebsormidium sp. (SCHW) suggests a relatively higher ability to tolerate lower light and this could be attributed to the morphological differences between the two strains. In fact, the longer and tangled filaments of Klebsormidium sp. (SCHW) in comparison to K. cf. flaccidum (KUE1) are expected to make cells of this isolate to occur in a comparatively more self-shaded environment. The Klebsormidium sp. (SCHW) also showed negative effects on F v /F m by low culture pH, excluding the possibility that this could be a different ecotype. We, therefore, suggest that Klebsormidium occurrence in ecological niches with stressful physiological conditions (i.e., low pH, potentially elevated heavy metal concentration) is favoured by the reduced competition with other species.
Although there are indications that some Klebsormidium isolates showed preferences for certain substrata with differing pH (Ryšánek et al. 2016).
The new Klebsormidium sp. (SCHW) isolate was also characterized by light-and transmission electron microscopy, to get further insights into the subcellular organization. Prominent pyrenoids, surrounded by numerous starch grains are observed. Like for Chlamydomonas, the thylakoid membrane traversing the pyrenoid could be involved in CCM activity by containing a lumenal carbonic anhydrase which aids the conversion of HCO 3 -(transported inside the lumen from the stroma) into CO 2 and deliver CO 2 in proximity to Rubisco (Moroney et al. 2011;Meyer and Griffiths 2013).
The occurrence of numerous plastoglobules in chloroplasts is usually an indication for thylakoid membrane degradation (e.g. Holzinger et al. 2011). One interesting observation was that the nuclei were frequently found in a position close to the cross cell walls. This is usually only the case after cell division (e.g., Lokhorst and Star 1985) and the nucleus moves then back to its central position. The frequent occurrence of this position either could point towards increased division activity or could be an indication of incomplete cell divisions.
For both Klebsormidium isolates and Zygnema the I k parameter was found to be low and very similar, suggesting a low light adaptation for these organisms Karsten et al. 2016). The presence of such low light adaptation contradicts the intuition that algae of the soil crusts (including those from high Alpine environments) may experience direct and intense solar radiation in natural conditions (Gray et al. 2007;Karsten et al. 2010). In contrast, the chlorophyte Chlorella ohadii isolated from highly irradiated desert soil crust can tolerate light intensities as high as 3500 lmol photons m -2 s -1 (Treves et al. 2013(Treves et al. , 2016. It has been suggested that low light adapted terrestrial species occur in micro-environments of the soil crust where they are protected from incident light, or might be relived from stressful light conditions by filaments selfshading (Gray et al. 2007;Karsten et al. 2016). A similar Despite the similar photosynthetic light characteristics between Klebsormidium and Zygnema, drastic differences were observed in their photoprotective mechanisms. Gerotto and Morosinotto (2013) described that for Klebsormidium and Zygnema the major component of NPQ is represented by the energy-dependent qE, which is regulated by lumen acidification (Roach and Krieger-Liszkay 2014). For Klebsormidium the maximal NPQ was higher than in Zygnema. Moreover, for this genus the NPQ was inducible and its full capacity was reached relatively slowly during exposure to strong light, particularly in comparison to other streptophyte algae (Gerotto and Morosinotto 2013) or some aquatic microalgae (Kotabová et al. 2011;La Rocca et al. 2015). Lumen acidification and NPQ induction may also involve the presence of an active cyclic electron flow around PSI (CEF-PSI) (Golding and Johnson 2003;Joliot and Johnson 2011), and whose activity has been shown in K. flaccidum (Hori et al. 2014). Contrary to Klebsormidium, Zygnema showed a lower and a more constitutive capacity to perform NPQ. Interestingly, Zygnema differs from Klebsormidium also for not having LHCSR involved NPQ activation but PSBS protein, resembling the NPQ activation in vascular plants (Gerotto and Morosinotto 2013). However, the presence of PSBS may not be necessarily related to the closer phylogenetic  position of Zygnema to these organisms (Christa et al. 2017).
The differences in NPQ capacity and kinetics are linked the different responses of Klebsormidium and Zygnema photosynthetic apparatus to slow or rapid increase of light intensity. When the light in the environment increased relatively slow (as during P vs I curves), the high NPQ capacity prevented the low light adapted photosynthetic apparatus of Klebsormidium from being photoinhibited, even at light intensities as high as 1500 lmol photons m -2 s -1 . However, due to the slow NPQ activation, the photosynthetic apparatus was prone to suffer from photoinhibition if the light in the environment increases rapidly (RLC curves). During the RLCs, the activation of NPQ under strong light may also explain the inflection of the slope of photoinhibition, particularly noticeable for K. cf. flaccidum (KUE1). From the eco-physiological point of view, these photosynthetic responses reflect the Klebsormidium adaptation to highly irradiated terrestrial environments where increases or changes of light intensity (related to solar position in the sky or cloud cover) occur slowly during the day. For Zygnema with a much lower and not inducible NPQ capacity, the presence of photoinhibition was found under both slow and rapid increase of light. This suggests that Zygnema could prefer environments with more shaded conditions than Klebsormidium. In addition to NPQ, presence of different UV protecting compounds as phenolics in Zygnema (Pichrtová et al. 2013) or mycosporine-like amino acids in Klebsormidium (Kitzing et al. 2014) may further modulate the resistance under natural light conditions.
For species of the soil crust, slow increases of light intensity during mornings can also be associated with dehydration (Raanan et al. 2016a). For the young Zygnema culture investigated in the present study, we found no resistance to desiccation as previously described . For Zygnema another strategy might be very important, the ability to form pre-akinetes that accumulate lipids , and showed a reduced physiological activity, beneficial to tolerated desiccation stress (e.g., Pichrtová et al. 2014). However, these pre-akinetes were not subject of the present study. In the case of the desiccation tolerant Klebsormidium, exposure to relatively high light intensity (our SL condition) during dehydration compromised the photosynthetic apparatus functioning, similarly to other terrestrial microalgae (Gray et al. 2007). Under dehydration in SL, the most noticeable change in PSII fluorescence signal was the increasing minimal F o . This result is analogous to what has been previously observed for the marine green macroalgae Ulva, where the F o increased during, at least for the first part, the dehydration experiment (Gao et al. 2011). The authors suggested that the F o increase is associated to a reversible inactivation of PSII reaction centres The parameter V j was calculated according to the equation of Strasser et al. (2000). Asterisks indicate statistically significant differences from the low light treatment or the separation of the antenna complex from the PSII. However, these alterations seem not to happen at PSII level in Klebsormidium since the F m values were not affected during dehydration. It is also known that F o is influenced by the dark reduction of the plastoquinone (PQ) pool (Groom et al. 1993;Stirbet et al. 2014). Complementary to higher F o , the OJIP analysis revealed an increase in the J step (V j ) with values as high as the P step (F m ), and such changes have been associated to a higher reduction PQ pool (Tóth et al. 2007). We, therefore, relate the increasing F o during dehydration in SL to an electron accumulation and a reduced state of the plastoquinone (PQ) pool along the electron transport chain (ETC). The progressive accumulation of electrons in the ETC under this condition, may eventually enhance radiative charge recombination events, leading to singlet oxygen production (Ohad et al. 2010(Ohad et al. , 2011 and thus, being responsible for the hastened PSII inactivation (YII decline) and impaired recovery after rehydration. It must also be pointed out that the SL used for this experiment is rather low (185 lmol photons m -2 s -1 ) compared to full sunlight intensity of an Alpine ecosystem. We also showed that under culture (i.e., hydrated) conditions Klebsormidium can tolerate up to eightfold this level of light intensity. We suggest that the reduction of the PQ (with the consequential damages on PSII) caused by relatively high light during dehydration events is an important physiological driver which shapes the species adaptation and occurrence towards low light. The (over) reduction of the ETC during dehydration in the SL could be linked to different physiological alterations. Holzinger et al. (2014) measured a decline in Klebsormidium CO 2 consumption rate during the dehydration phase. Thus, it is highly possible that electrons could be accumulated along the ETC following an imbalance between the excitation arriving at PSII, and the ability to remove electrons from the ETC, using them for CO 2 fixation (Shimakawa et al. 2017). In conjunction, alterations of mechanisms involved in the redox regulation of the PQ may also take place (Rumeau et al. 2007). It has been proposed that exposure to water loss and/or high light promotes the activity of CEF-PSI over the linear electron flow, aiding lumen acidification and inducing NPQ for PSII protection (Golding and Johnson 2003;Miyake et al. 2005;Gao et al. 2011;Meneghesso et al. 2016). Taking into consideration that a CEF-PSI (mediated through the NAD(P)H dehydrogenase complex) is possibly operative in Klebsormidium (Hori et al. 2014), it is reasonable to hypothesise that even for our Klebsormidium strain the activity CEF-PSI could be intensified under dehydration at SL, and thus contributing to PQ pool reduction.
Although Klebsormidium and Zygnema have distinguished light photosynthetic characteristics, we show that their photosynthetic C i acquisition is identical. The results of the pH-drift experiment reflected the cells ability to extract different C i forms from the water environment (Maberly and Spence 1983). The pH increase up to final values of *9.7 suggests that Klebsormidium and Zygnema are able to uptake both CO 2 and HCO 3 for photosynthesis, and thus providing further evidence that these organisms have functional CCMs (Maberly et al. 2009). The high C T /Alk quotients are similar to other CO 2 -users, as Chlamydomonas sp. and planktonic desmids (Zygnematophyceae; Spijkerman et al. 2005;Lachmann et al. 2016). This, however, could be an indication that both Klebsormidium and Zygnema have a preference for CO 2 . This would be particularly relevant for Klebsormidium whose occurrence is restricted to soil and aero-terrestrial environments, where HCO 3 is not available for photosynthesis. These results do not support our hypothesis that Klebsormidium and Zygnema with different restriction to water (or isolated from acidic environment as in Lachmann et al. 2016), might possess distinguished C i acquisition modes. Rather, they lead to the conclusion that these organisms acquired similar adaptations of their C i acquisition during land colonization. Further studies are necessary to fully describe the terrestrial adaptation of their CCMs. Terrestrial streptophyte species are expected to be sensitive to spatial and temporal variation of CO 2 in the environment. Spatially, CO 2 variations could be linked to soil respiration which, stimulated by flux of organic matter, represents an input of CO 2 (Suseela et al. 2012;Ng et al. 2014;Raven and Colmer 2016). Under the global change scenario, the long-term increase of atmospheric pCO 2 , predicted to reach values as high as 1000 ppmv by the end of twenty-first century (IPCC 2014), could cause genotypic changes (Collins and Bell 2004).
In conclusion, our work demonstrated that Klebsormidium and Zygnema possess distinguished photosynthetic traits which allow them to occur under different light regimes. These physiological traits might be the consequence of several adaptations acquired during their land colonization. Terrestrial environmental conditions as high irradiation and desiccation may have been counteracting forces shaping their photosynthetic apparatus. The high light may have favoured the acquisition of photoprotective mechanisms which allow them to occur in elevated light regimes (Alboresi et al. 2008(Alboresi et al. , 2010. Opposite, the alterations of the ETC (leading to PSII damages) during dehydration under illuminated conditions may have favoured a shade adaptation. This is reflected by organisms such as Klebsormidium with a low light adapted photosynthetic apparatus but tolerant to high light intensity. It is also interesting that Klebsormidium and Zygnema, from different locations, habitat preferences and evolutionary positions share a similar C i acquisition mode. It is known that atmospheric variations of CO 2 /O 2 through geological time have given origin to diverse CCMs in aquatic algae (Raven et al. 2012(Raven et al. , 2017Hagemann et al. 2016). Genetic and molecular characterizations of CCMs in streptophyte algae are currently missing in the literature, although these could provide a useful insight on how atmospheric CO 2 conditions have influenced land colonization by photosynthetic organisms.
Author contribution statement MP and AH designed the research and wrote the manuscript. MP conducted the physiological experiments. AH performed the light-and transmission electron microscopy. DR carried out the phylogenetic analysis. IL and WA collected and provided Klebsormidium sp. (Schwarzwand, Austria). All authors read and approved the manuscript.
Walsby AE (1997) Numerical integration of phytoplankton photosynthesis through time and depth in a water column. New Phytol 136:189-209 Yamakawa H, Fukushima Y, Itoh S, Heber U (2012) Three different mechanisms of energy dissipation of a desiccation-tolerant moss serve one common purpose: to protect reaction centres against photo-oxidation. J Exp Bot 63:1-11 Zwickl DJ (2006) Genetic algorithm approaches for the phylogenetic analysis of large biological sequence datasets under the maximum likelihood criterion. Dissertation, University of Texas at Austin