Titel
Human amniotic membrane as newly identified source of amniotic fluid pulmonary surfactant
Autor*in
Angela Lemke
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology / AUVA Research Center
José Carlos Castillo-Sánchez
Departamento de Bioquimica, Facultad de Biologia, and Instituto de Investigación Hospital Doce de Octubre, Universidad Complutense
... show all
Abstract
Pulmonary surfactant (PS) reduces surface tension at the air-liquid interface in the alveolar epithelium of the lung, which is required for breathing and for the pulmonary maturity of the developing foetus. However, the origin of PS had never been thoroughly investigated, although it was assumed to be secreted from the foetal developing lung. Human amniotic membrane (hAM), particularly its epithelial cell layer, composes the amniotic sac enclosing the amniotic fluid. In this study, we therefore aimed to investigate a potential contribution of the cellular components of the hAM to pulmonary surfactant found in amniotic fluid. We identified that cells within the native membrane contain lamellar bodies and express all four surfactant proteins as well as ABCA3. Lipidomic profiling by nanoESI – MS/MS revealed the presence of the essential lipid species as found in PS. Also, the biophysical activity of conditioned cell culture supernatant obtained from hAM was tested with captive bubble surfactometry. hAM supernatant showed the ability to reduce surface tension, similar to human PS obtained from bronchoalveolar lavage. This means that hAM produces the essential PS-associated components and can therefore contribute as second potential source of PS in amniotic fluid aside from the foetal lung.
Stichwort
Cell biologyMolecular biology
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
Scientific Reports
Band
7
Publication
Springer Nature
Erscheinungsdatum
2017
Zugänglichkeit
Rechte
Rechteangabe
© The Author(s) 2017
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