Titel
GC Content of Early Metazoan Genes and Its Impact on Gene Expression Levels in Mammalian Cell Lines
Autor*in
Ismail Sahin Gul
Center for Inflammation Research, Flanders Institute for Biotechnology (VIB)
Autor*in
Jens Staal
Center for Inflammation Research, Flanders Institute for Biotechnology (VIB)
Autor*in
Paco Hulpiau
Center for Inflammation Research, Flanders Institute for Biotechnology (VIB)
... show all
Abstract
With the genomes available for many animal clades, including the early-branching metazoans, one can readily study the functional conservation of genes across a diversity of animal lineages. Ectopic expression of an animal protein in, for instance, a mammalian cell line is a generally used strategy in structure–function analysis. However, this might turn out to be problematic in case of distantly related species. Here we analyzed the GC content of the coding sequences of basal animals and show its impact on gene expression levels in human cell lines, and, importantly, how this expression efficiency can be improved. Optimization of the GC3 content in the coding sequences of cadherin, alpha-catenin, and paracaspase of Trichoplax adhaerens dramatically increased the expression of these basal animal genes in human cell lines.
Stichwort
Trichoplax adhaerensPlacozoagenome evolutionGC contentectopic expression
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
https://phaidra.univie.ac.at/o:1045195
Erschienen in
Titel
Genome Biology and Evolution
Band
10
Ausgabe
3
Seitenanfang
909
Seitenende
917
Verlag
Oxford University Press (OUP)
Erscheinungsdatum
2018
Zugänglichkeit
Rechteangabe
© The Author(s) 2018

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