This study on research collaboration (RC) is an attempt to estimate the degree of internationalization of academic institutions and regions. Furthermore potential influences of RC on excellence initiatives of modern universities are investigated relying on source data obtained from SCImago Institutions Rankings (SIR). A positive correlation exists between the degree of collaboration and the normalized impact. However, in contrast to output the normalized impact increase progression is non-linear and fluctuating. Differences occur regarding output volume and normalized impact at geographical region level for the leading universities. Different patterns of the Brute Force distribution for each collaboration type were are also observed at region level as well as at subject area level. A continuously reduced percentage of the domestic (non-collaboration) academic output is a world trend, whereas a steady increase of “international + national” collaboration is observed globally, however, less distinctive in Asia than in the other regions. The impact of Latin American papers originating from domestic production as well as from national collaboration remains considerably below world average values.