Titel
Impact of Hfq on the Bacillus subtilis Transcriptome
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Abstract
The RNA chaperone Hfq acts as a central player in post-transcriptional gene regulation in several Gram-negative Bacteria, whereas comparatively little is known about its role in Gram-positive Bacteria. Here, we studied the function of Hfq in Bacillus subtilis, and show that it confers a survival advantage. A comparative transcriptome analysis revealed mRNAs with a differential abundance that are governed by the ResD-ResE system required for aerobic and anaerobic respiration. Expression of resD was found to be up-regulated in the hfq− strain. Furthermore, several genes of the GerE and ComK regulons were de-regulated in the hfq− background. Surprisingly, only six out of >100 known and predicted small RNAs (sRNAs) showed altered abundance in the absence of Hfq. Moreover, Hfq positively affected the transcript abundance of genes encoding type I toxin-antitoxin systems. Taken the moderate effect on sRNA levels and mRNAs together, it seems rather unlikely that Hfq plays a central role in RNA transactions in Bacillus subtilis.
Stichwort
Bacillus subtilisMessenger RNADNA transcriptionRegulonsRNA extractionGene regulationRNA annealingPlasmid construction
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
https://phaidra.univie.ac.at/o:471745
Erschienen in
Titel
PLoS ONE
Band
9
Ausgabe
6
Verlag
Public Library of Science (PLoS)
Erscheinungsdatum
2014
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