• A comparative study of five physiological key parameters between four different human trophoblast-derived cell lines

    • Mario Rothbauer
      Institute of Applied Synthetic Chemistry & Institute of Chemical Technologies and Analytics, Faculty of Technical Chemistry, Vienna University of Technology
    • Nilaykumar Patel
      Department für Pharmakognosie, Fakultät für Lebenswissenschaften, Universität Wien
    • Hajnalka Gondola
      Institute of Applied Synthetic Chemistry & Institute of Chemical Technologies and Analytics, Faculty of Technical Chemistry, Vienna University of Technology
    • Monika Siwetz
      Institute of Cell Biology, Histology and Embryology, Medical University of Graz
    • Berthold Huppertz
      Institute of Cell Biology, Histology and Embryology, Medical University of Graz
    • Peter Ertl
      Institute of Applied Synthetic Chemistry & Institute of Chemical Technologies and Analytics, Faculty of Technical Chemistry, Vienna University of Technology
  • The human placenta plays a crucial role as the interface between mother and fetus. It represents a unique tissue that undergoes morphological as well as functional changes on the cellular and tissue level throughout pregnancy. To better understand how the placenta works, a variety of techniques has been developed to re-create this complex physiological barrier in vitro. However, due to the low availability of freshly isolated primary cells, choriocarcinoma cell lines remain the usual suspects as in vitro models for placental research. Here, we present a comparative study on the functional aspects of the choriocarcinoma cell lines BeWo, JAR and Jeg-3, as well as the first trimester trophoblast cell line ACH-3P as placental in vitro barrier models for endocrine and transport studies. Functional assays including tight junction immunostaining, sodium fluorescein retardation, trans epithelial resistance, glucose transport, hormone secretion as well as size-dependent polystyrene nanoparticle transport were performed using the four cell types to evaluate key functional parameters of each cell line to act a relevant in vitro placental barrier model.

  • PDF

  • http://phaidra.univie.ac.at/o:918512

  • Wissenschaftlicher Artikel

  • Veröffentlichte Version

  • Scientific Reports

  • 2017

  • 7

  • Springer Nature

  • Englisch

  • Frei zugänglich

  • CC BY Namensnennung 4.0 International
    © The Author(s) 2017

  • 685817 – Europäische Union (alle Programme)

  • 2045-2322

  • Biological models; Nanobiotechnology