Titel
In-situ visualization of sound-induced otolith motion using hard X-ray phase contrast imaging
Autor*in
Tanja Schulz-Mirbach
Zoology, Department Biology II, Ludwig-Maximilians-University Munich
Autor*in
Margie Olbinado
European Synchrotron Radiation Facility (ESRF, ID19)
Autor*in
Alexander Rack
European Synchrotron Radiation Facility (ESRF, ID19)
... show all
Abstract
Regarding the basics of ear structure-function relationships in fish, the actual motion of the solid otolith relative to the underlying sensory epithelium has rarely been investigated. Otolith motion has been characterized based on a few experimental studies and on approaches using mathematical modeling, which have yielded partially conflicting results. Those studies either predicted a simple back-and-forth motion of the otolith or a shape-dependent, more complex motion. Our study was designed to develop and test a new set-up to generate experimental data on fish otolith motion in-situ. Investigating the basic parameters of otolith motion requires an approach with high spatial and temporal resolution. We therefore used hard X-ray phase contrast imaging (XPCI). We compared two anatomically well-studied cichlid species, Steatocranus tinanti and Etroplus maculatus, which, among other features, differ in the 3D shape of their otoliths. In a water-filled tank, we presented a pure tone of 200 Hz to 1) isolated otoliths embedded in agarose serving as a simple model or 2) to a fish (otoliths in-situ). Our new set-up successfully visualized the motion of otoliths in-situ and therefore paves the way for future studies evaluating the principles of otolith motion.
Stichwort
Animal physiologyInner earNeurophysiology
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
https://phaidra.univie.ac.at/o:931086
Erschienen in
Titel
Scientific Reports
Band
8
Verlag
Springer Nature
Erscheinungsdatum
2018
Zugänglichkeit
Rechteangabe
© The Author(s) 2018

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