Title
Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in Sturgeon
Author
Abdul Rasheed Baloch
South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice
Michaela Fučíková
South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice
Marek Rodina
South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice
... show all
Abstract
Nanoparticles are finding increasing applications in diagnostics, imaging and therapeutics in medicine. Iron oxide nanoparticles (IONs) have received significant interest of scientific community due to their distinctive properties. For the first time, we have delivered IONs into germ cells in any species. Our results showed that sturgeon primordial germ cells (PGCs) delivered with IONs could be detected until seven days post fertilization (dpf) under fluorescent microscope and at 22 dpf by micro-CT. Delivery of IONs into cells could be helpful for studying germ cell biology and the improvement of germ cell-based bio-technologies as isolation of PGCs using magnetic activated cell sorting or application of hyperthermia for a host sterilization purpose. Intriguingly, in our study, we did not find any toxic effects of IONs on the survival and hatching rates of sturgeon embryos when compared with embryos injected with FITC-dextran only.
Keywords
acipensercaviarhyperthermiairon oxide nanoparticlesmicro-CTsterilization
Object type
Language
English [eng]
Persistent identifier
phaidra.univie.ac.at/o:1141821
Appeared in
Title
Biomolecules
Volume
9
Issue
8
ISSN
2218-273X
Issued
2019
Publication
MDPI AG
Version type
Date issued
2019
Access rights
Rights
Rights statement
© 2019 by the authors
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