Titel
A scalable culturing system for the marine annelid Platynereis dumerilii
Autor*in
Emily Kuehn
Marine Biological Laboratory
Alexander W. Stockinger
Jerome Girard
Marine Biological Laboratory
... show all
Abstract
Platynereis dumerilii is a marine segmented worm (annelid) with externally fertilized embryos and it can be cultured for the full life cycle in the laboratory. The accessibility of embryos and larvae combined with the breadth of the established molecular and functional techniques has made P. dumerilii an attractive model for studying development, cell lineages, cell type evolution, reproduction, regeneration, the nervous system, and behavior. Traditionally, these worms have been kept in rooms dedicated for their culture. This allows for the regulation of temperature and light cycles, which is critical to synchronizing sexual maturation. However, regulating the conditions of a whole room has limitations, especially if experiments require being able to change culturing conditions. Here we present scalable and flexible culture methods that provide ability to control the environmental conditions, and have a multi-purpose culture space. We provide a closed setup shelving design with proper light conditions necessary for P. dumerilii to mature. We also implemented a standardized method of feeding P. dumerilii cultures with powdered spirulina which relieves the ambiguity associated with using frozen spinach, and helps standardize nutrition conditions across experiments and across different labs. By using these methods, we were able to raise mature P. dumerilii, capable of spawning and producing viable embryos for experimentation and replenishing culture populations. These methods will allow for the further accessibility of P. dumerilii as a model system, and they can be adapted for other aquatic organisms.
Stichwort
SunlightLarvaeAlgaeEmbryosSea waterSpinachFertilizationLight
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
PLOS ONE
Band
14
Ausgabe
12
ISSN
1932-6203
Erscheinungsdatum
2019
Publication
Public Library of Science (PLoS)
Erscheinungsdatum
2019
Zugänglichkeit
Rechte
Rechteangabe
© 2019 Kuehn et al.
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