Titel
Targeted Phosphoinositides Analysis Using High-Performance Ion Chromatography-Coupled Selected Reaction Monitoring Mass Spectrometry
Autor*in
Philipp Westhoff
Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V.
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Abstract
Phosphoinositides are minor components of cell membranes, but play crucial roles in numerous signal transduction pathways. To obtain quantitative measures of phosphoinositides, sensitive, accurate, and comprehensive methods are needed. Here, we present a quantitative targeted ion chromatography–mass spectrometry-based workflow that separates phosphoinositide isomers and increases the quantitative accuracy of measured phosphoinositides. Besides testing different analytical characteristics such as extraction and separation efficiency, the reproducibility of the developed workflow was also investigated. The workflow was verified in resting and stimulated human platelets, fat cells, and rat hippocampal brain tissue, where the LOD and LOQ for phosphoinositides were at 312.5 and 625 fmol, respectively. The robustness of the workflow is shown with different applications that confirms its suitability to analyze multiple less-abundant phosphoinositides.
Stichwort
phosphoinositidestargeted lipidomicsion chromatography
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
Journal of Proteome Research
Band
20
Ausgabe
6
ISSN
1535-3893
Erscheinungsdatum
2021
Seitenanfang
3114
Seitenende
3123
Publication
American Chemical Society (ACS)
Erscheinungsdatum
2021
Zugänglichkeit
Rechte
Rechteangabe
© 2021 The Authors
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