Titel
Multiomics of synaptic junctions reveals altered lipid metabolism and signaling following environmental enrichment
Autor*in
Maximilian Borgmeyer
Leibniz Group ‘Dendritic Organelles and Synaptic Function,’ University Medical Center Hamburg-Eppendorf
Canan Has
Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V.
... show all
Abstract
Membrane lipids and their metabolism have key functions in neurotransmission. Here we provide a quantitative lipid inventory of mouse and rat synaptic junctions. To this end, we developed a multiomics extraction and analysis workflow to probe the interplay of proteins and lipids in synaptic signal transduction from the same sample. Based on this workflow, we generate hypotheses about novel mechanisms underlying complex changes in synaptic connectivity elicited by environmental stimuli. As a proof of principle, this approach reveals that in mice exposed to an enriched environment, reduced endocannabinoid synthesis and signaling is linked to increased surface expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) in a subset of Cannabinoid-receptor 1 positive synapses. This mechanism regulates synaptic strength in an input-specific manner. Thus, we establish a compartment-specific multiomics workflow that is suitable to extract information from complex lipid and protein networks involved in synaptic function and plasticity.
Stichwort
Lipidomicssynaptic junctionsendocannabinoid signalingmultiomicsenriched environment
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
Cell Reports
Band
37
Ausgabe
1
ISSN
2211-1247
Erscheinungsdatum
2021
Publication
Elsevier BV
Erscheinungsdatum
2021
Zugänglichkeit
Rechteangabe
© 2021 The Author(s)
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