Titel
Isolation and Characterization of Cell Envelope Fragments Comprising Archaeal S-Layer Proteins
Autor*in
Kevin Pfeifer
Institute of Synthetic Bioarchitectures, University of Natural Resources and Life Sciences
Eva-Kathrin Ehmoser
Institute of Synthetic Bioarchitectures, University of Natural Resources and Life Sciences
... show all
Abstract
The outermost component of cell envelopes of most bacteria and almost all archaea comprise a protein lattice, which is termed Surface (S-)layer. The S-layer lattice constitutes a highly porous structure with regularly arranged pores in the nm-range. Some archaea thrive in extreme milieus, thus producing highly stable S-layer protein lattices that aid in protecting the organisms. In the present study, fragments of the cell envelope from the hyperthermophilic acidophilic archaeon Saccharolobus solfataricus P2 (SSO) have been isolated by two different methods and characterized. The organization of the fragments and the molecular sieving properties have been elucidated by transmission electron microscopy and by determining the retention efficiency of proteins varying in size, respectively. The porosity of the archaeal S-layer fragments was determined to be 45%. S-layer fragments of SSO showed a retention efficiency of up to 100% for proteins having a molecular mass of ≥ 66 kDa. Moreover, the extraction costs for SSO fragments have been reduced by more than 80% compared to conventional methods, which makes the use of these archaeal S-layer material economically attractive.
Stichwort
archaeaS-layer proteinArchaea Biotechnologymolecular sievingextraction methodbiomimetics
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
Nanomaterials
Band
12
Ausgabe
14
ISSN
2079-4991
Erscheinungsdatum
2022
Publication
MDPI AG
Erscheinungsdatum
2022
Zugänglichkeit
Rechte
Rechteangabe
© 2022 by the authors
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