Titel
Throttling Growth Speed: Evaluation of aux1-7 Root Growth Profile by Combining D-Root system and Root Penetration Assay
Autor*in
Judith García-González
Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, Czech Academy of Sciences
Jozef Lacek
Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, Czech Academy of Sciences
... show all
Abstract
Directional root growth control is crucial for plant fitness. The degree of root growth deviation depends on several factors, whereby exogenous growth conditions have a profound impact. The perception of mechanical impedance by wild-type roots results in the modulation of root growth traits, and it is known that gravitropic stimulus influences distinct root movement patterns in concert with mechanoadaptation. Mutants with reduced shootward auxin transport are described as being numb towards mechanostimulus and gravistimulus, whereby different growth conditions on agar-supplemented medium have a profound effect on how much directional root growth and root movement patterns differ between wild types and mutants. To reduce the impact of unilateral mechanostimulus on roots grown along agar-supplemented medium, we compared the root movement of Col-0 and auxin resistant 1-7 in a root penetration assay to test how both lines adjust the growth patterns of evenly mechanostimulated roots. We combined the assay with the D-root system to reduce light-induced growth deviation. Moreover, the impact of sucrose supplementation in the growth medium was investigated because exogenous sugar enhances root growth deviation in the vertical direction. Overall, we observed a more regular growth pattern for Col-0 but evaluated a higher level of skewing of aux1-7 compared to the wild type than known from published data. Finally, the tracking of the growth rate of the gravistimulated roots revealed that Col-0 has a throttling elongation rate during the bending process, but aux1-7 does not.
Stichwort
AUXIN-RESISTANT 1AUX1directional root growthgravitropic responsemechanostimulusmechanoadaptationroot skewingroot elongation rateD-root systemroot penetration assay
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
Plants
Band
11
Ausgabe
5
ISSN
2223-7747
Erscheinungsdatum
2022
Publication
MDPI AG
Erscheinungsdatum
2022
Zugänglichkeit
Rechte
Rechteangabe
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