Titel
Single-cell stable isotope probing in microbial ecology
Autor*in
Uria Alcolombri
Institute of Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering, ETH Zurich
Roberto Pioli
Institute of Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering, ETH Zurich
Roman Stocker
Institute of Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering, ETH Zurich
... show all
Abstract
Environmental and host-associated microbiomes are typically diverse assemblages of organisms performing myriad activities and engaging in a network of interactions that play out in spatially structured contexts. As the sum of these activities and interactions give rise to overall microbiome function, with important consequences for environmental processes and human health, elucidating specific microbial activities within complex communities is a pressing challenge. Single-cell stable isotope probing (SC-SIP) encompasses multiple techniques that typically utilize Raman microspectroscopy or nanoscale secondary ion mass spectrometry (NanoSIMS) to enable spatially resolved tracking of isotope tracers in cells, cellular components, and metabolites. SC-SIP techniques are uniquely suited for illuminating single-cell activities in microbial communities and for testing hypotheses about cellular functions generated for example from meta-omics datasets. Here, we illustrate the insights enabled by SC-SIP techniques by reviewing selected applications in microbiology and offer a perspective on their potential for future research.
Stichwort
Environmental microbiologyStable isotope analysis
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
ISME Communications
Band
2
ISSN
2730-6151
Erscheinungsdatum
2022
Publication
Springer Science and Business Media LLC
Erscheinungsdatum
2022
Zugänglichkeit
Rechte
Rechteangabe
© The Author(s) 2022
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