Titel
CT295 Is Chlamydia trachomatis’ Phosphoglucomutase and a Type 3 Secretion Substrate
Autor*in
Sébastien Triboulet
Institut Pasteur, Université Paris Cité
Maimouna D. N’Gadjaga
Institut Pasteur, Université Paris Cité
Béatrice Niragire
Institut Pasteur, Université Paris Cité
... show all
Abstract
The obligate intracellular bacteria Chlamydia trachomatis store glycogen in the lumen of the vacuoles in which they grow. Glycogen catabolism generates glucose-1-phosphate (Glc1P), while the bacteria can take up only glucose-6-phosphate (Glc6P). We tested whether the conversion of Glc1P into Glc6P could be catalyzed by a phosphoglucomutase (PGM) of host or bacterial origin. We found no evidence for the presence of the host PGM in the vacuole. Two C. trachomatis proteins, CT295 and CT815, are potential PGMs. By reconstituting the reaction using purified proteins, and by complementing PGM deficient fibroblasts, we demonstrated that only CT295 displayed robust PGM activity. Intriguingly, we showed that glycogen accumulation in the lumen of the vacuole of a subset of Chlamydia species (C. trachomatis, C. muridarum, C. suis) correlated with the presence, in CT295 orthologs, of a secretion signal recognized by the type three secretion (T3S) machinery of Shigella. C. caviae and C. pneumoniae do not accumulate glycogen, and their CT295 orthologs lack T3S signals. In conclusion, we established that the conversion of Glc1P into Glc6P was accomplished by a bacterial PGM, through the acquisition of a T3S signal in a “housekeeping” protein. Acquisition of this signal likely contributed to shaping glycogen metabolism within Chlamydiaceae.
Stichwort
Chlamydiaglycogenmetabolismtype 3 secreted effectorsphosphoglucomutase (PGM)secretion signal
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
Frontiers in Cellular and Infection Microbiology
Band
12
ISSN
2235-2988
Erscheinungsdatum
2022
Publication
Frontiers Media SA
Erscheinungsdatum
2022
Zugänglichkeit
Rechte
Rechteangabe
© 2022 Triboulet, N’Gadjaga, Niragire, Köstlbacher, Horn, Aimanianda and Subtil
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