Titel
Heat-induced proteomic changes in anthers of contrasting rice genotypes under variable stress regimes
Autor*in
Ritesh Kumar
Department of Plant Molecular Biology, University of Delhi
Isha Goyal
Department of Plant Molecular Biology, University of Delhi
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Abstract
Heat stress drastically affects anther tissues resulting in poor plant fertility, necessitating an urgent need to determine the key proteome regulation associated with mature anther in response to heat stress. We identified several genotype - specific protein alterations in rice anthers of Moroberekan (Japonica, heat sensitive), IR64 (Indica, moderately heat tolerant), and Nagina22 (Aus, heat tolerant) in the short-term (ST_HS; one cycle of 42°C, 4 hours before anthesis) and long-term (LT_HS; 6 cycles of 38°C, 6 hours before anthesis) heat stress. The proteins upregulated in long-term heat stress in Nagina22 were enriched in biological processes related to unfolded protein binding and carboxylic acid metabolism, including amino acid metabolism. In short-term heat stress, Nagina22 anthers were enriched in proteins associated with vitamin E biosynthesis and GTPase activator activity. In contrast, downregulated proteins were related to ribosomal proteins. The expression of different Hsp20 and DnaJ was genotype specific. Overall, the heat response in Nagina22 was associated with its capacity for adequate metabolic control and cellular homeostasis, which may be critical for its higher reproductive thermotolerance. This study improves our understanding of thermotolerance mechanisms in rice anthers during anthesis and lays a foundation for breeding thermotolerant varieties via molecular breeding.
Stichwort
anthersheat stressLC-MS/MSproteomicsreproductive thermotolerancerice
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
Frontiers in Plant Science
Band
13
ISSN
1664-462X
Erscheinungsdatum
2023
Publication
Frontiers Media SA
Erscheinungsdatum
2023
Zugänglichkeit
Rechte
Rechteangabe
© 2023 Kumar, Ghatak, Goyal, Sarkar, Weckwerth, Grover and Chaturvedi
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