Titel
Targeting the NF-κB p65/Bcl-2 signaling pathway in hepatic cellular carcinoma using radiation assisted synthesis of zinc nanoparticles coated with naturally isolated gallic acid
Autor*in
Omayma A.R. AboZaid
Department of Biochemistry, Faculty of Veterinary Medicine, Moshtohor, Benha University
Autor*in
Mostafa A. Abdel-Maksoud
Botany and Microbiology department, College of Science, King Saud University
Autor*in
Ibrahim A. Saleh
Faculty of Science, Zarqa University
... show all
Abstract
Purpose: Oral diethylnitrosamine (DEN) is a known hepatocarcinogen that damages the liver and causes cancer. DEN damages the liver through reactive oxygen species-mediated inflammation and biological process regulation. Materials and methods: Gallic acid-coated zinc oxide nanoparticles (Zn-GANPs) were made from zinc oxide (ZnO) synthesized by irradiation dose of 50 kGy utilizing a Co-60 γ-ray source chamber with a dose rate of 0.83 kGy/h and gallic acid from pomegranate peel. UV–visible (UV) spectrophotometry verified Zn-GANP synthesis. TEM, DLS, and FTIR were utilized to investigate ZnO-NPs' characteristics. Rats were orally exposed to DEN for 8 weeks at 20 mg/kg five times per week, followed by intraperitoneal injection of Zn-GANPs at 20 mg/kg for 5 weeks. Using oxidative stress, anti-inflammatory, liver function, histologic, apoptotic, and cell cycle parameters for evaluating Zn-GANPs treatment. Results: DEN exposure elevated inflammatory markers (AFP and NF-κB p65), transaminases (AST, ALT), γ-GT, globulin, and total bilirubin, with reduced protein and albumin levels. It also increased MDA levels, oxidative liver cell damage, and Bcl-2, while decreasing caspase-3 and antioxidants like GSH, and CAT. Zn-GANPs significantly mitigated these effects and lowered lipid peroxidation, AST, ALT, and γ-GT levels, significantly increased CAT and GSH levels (p<0.05). Zn-GANPs caused S and G2/M cell cycle arrest and G0/G1 apoptosis. These results were associated with higher caspase-3 levels and lower Bcl-2 and TGF-β1 levels. Zn-GANPs enhance and restore the histology and ultrastructure of the liver in DEN-induced rats. Conclusion: The data imply that Zn-GANPs may prevent and treat DEN-induced liver damage and carcinogenesis.
Stichwort
Hepatic carcinomaPomegranate peelGallic acidRadiationZn-GANPsNF-κB p65Bcl-2G2/MTGF-β1
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
https://phaidra.univie.ac.at/o:2083417
Erschienen in
Titel
Biomedicine & Pharmacotherapy
Band
172
ISSN
0753-3322
Erscheinungsdatum
2024
Verlag
Elsevier BV
Erscheinungsdatum
2024
Zugänglichkeit
Rechteangabe
© 2024 The Author(s)

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