Titel
MUT-7 exoribonuclease activity and localization are mediated by an ancient domain
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Abstract
The MUT-7 family of 3′–5′ exoribonucleases is evolutionarily conserved across the animal kingdom and plays essential roles in small RNA production in the germline. Most MUT-7 homologues carry a C-terminal domain of unknown function named MUT7-C appended to the exoribonuclease domain. Our analysis shows that the MUT7-C is evolutionary ancient, as a minimal version of the domain exists as an individual protein in prokaryotes. In animals, MUT7-C has acquired an insertion that diverged during evolution, expanding its functions. Caenorhabditis elegans MUT-7 contains a specific insertion within MUT7-C, which allows binding to MUT-8 and, consequently, MUT-7 recruitment to germ granules. In addition, in C. elegans and human MUT-7, the MUT7-C domain contributes to RNA binding and is thereby crucial for ribonuclease activity. This RNA-binding function most likely represents the ancestral function of the MUT7-C domain. Overall, this study sheds light on MUT7-C and assigns two functions to this previously uncharacterized domain.
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
Nucleic Acids Research
Band
52
Ausgabe
15
ISSN
0305-1048
Erscheinungsdatum
2024
Seitenanfang
9076
Seitenende
9091
Publication
Oxford University Press (OUP)
Erscheinungsdatum
2024
Zugänglichkeit
Rechte
Rechteangabe
© The Author(s) 2024
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