Title
Sororin is an evolutionary conserved antagonist of WAPL
Author
Marta Galova
Research Institute of Molecular Pathology (IMP)
Author
Alexander Schleiffer
Research Institute of Molecular Pathology (IMP)
... show all
Abstract
Cohesin mediates sister chromatid cohesion to enable chromosome segregation and DNA damage repair. To perform these functions, cohesin needs to be protected from WAPL, which otherwise releases cohesin from DNA. It has been proposed that cohesin is protected from WAPL by SORORIN. However, in vivo evidence for this antagonism is missing and SORORIN is only known to exist in vertebrates and insects. It is therefore unknown how important and widespread SORORIN’s functions are. Here we report the identification of SORORIN orthologs in Schizosaccharomyces pombe (Sor1) and Arabidopsis thaliana (AtSORORIN). sor1Δ mutants display cohesion defects, which are partially alleviated by wpl1Δ. Atsororin mutant plants display dwarfism, tissue specific cohesion defects and chromosome mis-segregation. Furthermore, Atsororin mutant plants are sterile and separate sister chromatids prematurely at anaphase I. The somatic, but not the meiotic deficiencies can be alleviated by loss of WAPL. These results provide in vivo evidence for SORORIN antagonizing WAPL, reveal that SORORIN is present in organisms beyond the animal kingdom and indicate that it has acquired tissue specific functions in plants.
Keywords
Chromosome segregationPlant cell biology
Object type
Language
English [eng]
Persistent identifier
https://phaidra.univie.ac.at/o:2092486
Appeared in
Title
Nature Communications
Volume
15
ISSN
2041-1723
Issued
2024
Publisher
Springer Science and Business Media LLC
Date issued
2024
Access rights
Rights statement
© The Author(s) 2024

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