Titel
Probing Native Protein Structures by Chemical Cross-linking, Mass Spectrometry, and Bioinformatics
Autor*in
Thomas Walzthoeni
ETH Zürich - Eidgenössische Technische Hochschule Zürich (ETH Zürich)
Abdullah Kahraman
ETH Zürich - Eidgenössische Technische Hochschule Zürich (ETH Zürich)
... show all
Abstract
Chemical cross-linking of reactive groups in native proteins and protein complexes in combination with the identification of cross-linked sites by mass spectrometry has been in use for more than a decade. Recent advances in instrumentation, cross-linking protocols, and analysis software have led to a renewed interest in this technique, which promises to provide important information about native protein structure and the topology of protein complexes. In this article, we discuss the critical steps of chemical cross-linking and its implications for (structural) biology: reagent design and cross-linking protocols, separation and mass spectrometric analysis of cross-linked samples, dedicated software for data analysis, and the use of cross-linking data for computational modeling. Finally, the impact of protein cross-linking on various biological disciplines is highlighted.
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
phaidra.univie.ac.at/o:245243
Erschienen in
Titel
Molecular and Cellular Proteomics
Band
9
Seitenanfang
1634
Seitenende
1649
Erscheinungsdatum
01.12.2010
Zugänglichkeit
Rechte
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