Titel
Insulin-Like Growth Factor I (IGF-1) Ec/Mechano Growth Factor - A Splice Variant of IGF-1 within the Growth Plate
Autor*in
Werner Schlegel
Department of Paediatrics and Adolescent Medicine, Medical University of Vienna
Adalbert Raimann
Department of Paediatrics and Adolescent Medicine, Medical University of Vienna
Daniel Halbauer
Department of Paediatrics and Adolescent Medicine, Medical University of Vienna
... show all
Abstract
Human insulin-like growth factor 1 Ec (IGF-1Ec), also called mechano growth factor (MGF), is a splice variant of insulin-like growth factor 1 (IGF-1), which has been shown in vitro as well as in vivo to induce growth and hypertrophy in mechanically stimulated or damaged muscle. Growth, hypertrophy and responses to mechanical stimulation are important reactions of cartilaginous tissues, especially those in growth plates. Therefore, we wanted to ascertain if MGF is expressed in growth plate cartilage and if it influences proliferation of chondrocytes, as it does in musculoskeletal tissues. MGF expression was analyzed in growth plate and control tissue samples from piglets aged 3 to 6 weeks. Furthermore, growth plate chondrocyte cell culture was used to evaluate the effects of the MGF peptide on proliferation. We showed that MGF is expressed in considerable amounts in the tissues evaluated. We found the MGF peptide to be primarily located in the cytoplasm, and in some instances, it was also found in the nucleus of the cells. Addition of MGF peptides was not associated with growth plate chondrocyte proliferation.
Stichwort
Growth plateChondrocytesMechano growth factorInsulin-like growth factorsLasersPolymerase chain reactionSwineSignal peptides
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
PLoS ONE
Band
8
Ausgabe
10
Publication
Public Library of Science (PLoS)
Erscheinungsdatum
2013
Zugänglichkeit
Rechte
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