Titel
High-density lipoprotein sensor based on molecularly imprinted polymer
Autor*in
Roongnapa Suedee
Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University
Abstract
Decreased blood level of high-density lipoprotein (HDL) is one of the essential criteria in diagnosing metabolic syndrome associated with the development of atherosclerosis and coronary heart disease. Herein, we report the synthesis of a molecularly imprinted polymer (MIP) that selectively binds HDL, namely, HDL-MIP, and thus serves as an artificial, biomimetic sensor layer. The optimized polymer contains methacrylic acid and N-vinylpyrrolidone in the ratio of 2:3, cross-linked with ethylene glycol dimethacrylate. On 10 MHz dual electrode quartz crystal microbalances (QCM), such HDL-MIP revealed dynamic detection range toward HDL standards in the clinically relevant ranges of 2–250 mg/dL HDL cholesterol (HDL-C) in 10 mM phosphate-buffered saline (PBS, pH = 7.4) without significant interference: low-density lipoprotein (LDL) yields 5% of the HDL signal, and both very-low-density lipoprotein (VLDL) and human serum albumin (HSA) yield 0%. The sensor reveals recovery rates between 94 and 104% at 95% confidence interval with precision of 2.3–7.7% and shows appreciable correlation (R 2 = 0.97) with enzymatic colorimetric assay, the standard in clinical tests. In contrast to the latter, it achieves rapid results (10 min) during one-step analysis without the need for sample preparation.
Stichwort
High-density lipoproteinMolecularly imprinted polymerQuartz crystal microbalanceLipoprotein sensor
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
Erschienen in
Titel
Analytical and Bioanalytical Chemistry
Band
410
Ausgabe
3
Seitenanfang
875
Seitenende
883
Publication
Springer Nature
Erscheinungsdatum
2017
Zugänglichkeit
Rechte
Rechteangabe
© The Author(s)
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