Title
PPARγ Modulates Long Chain Fatty Acid Processing in the Intestinal Epithelium
Author
Matej Oresic
Turku Centre for Biotechnology, University of Turku and Åbo Akademi University
Author
Cedric Le May
Institut du Thorax, INSERM, CNRS, UNIV Nantes
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Abstract
Nuclear receptor PPARγ affects lipid metabolism in several tissues, but its role in intestinal lipid metabolism has not been explored. As alterations have been observed in the plasma lipid profile of ad libitum fed intestinal epithelium-specific PPARγ knockout mice (iePPARγKO), we submitted these mice to lipid gavage challenges. Within hours after gavage with long chain unsaturated fatty acid (FA)-rich canola oil, the iePPARγKO mice had higher plasma free FA levels and lower gastric inhibitory polypeptide levels than their wild-type (WT) littermates, and altered expression of incretin genes and lipid metabolism-associated genes in the intestinal epithelium. Gavage with the medium chain saturated FA-rich coconut oil did not result in differences between the two genotypes. Furthermore, the iePPARγKO mice did not exhibit defective lipid uptake and stomach emptying; however, their intestinal transit was more rapid than in WT mice. When fed a canola oil-rich diet for 4.5 months, iePPARγKO mice had higher body lean mass than the WT mice. We conclude that intestinal epithelium PPARγ is activated preferentially by long chain unsaturated FAs compared to medium chain saturated FAs. Furthermore, we hypothesize that the iePPARγKO phenotype originates from altered lipid metabolism and release in epithelial cells, as well as changes in intestinal motility.
Keywords
PPARγintestinelipid metabolism
Object type
Language
English [eng]
Persistent identifier
https://phaidra.univie.ac.at/o:876235
Appeared in
Title
International Journal of Molecular Sciences
Volume
18
Issue
12
From page
2559
Publisher
MDPI AG
Date issued
2017
Access rights
Rights statement
© 2017 by the authors
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