Negative Control of RpoS Synthesis by the sRNA ReaL in Pseudomonas aeruginosa

Hue Thi Bach Nguyen; David Romero A.; Fabian Amman; Theresa Sorger-Domenigg; Muralidhar Tata; Elisabeth Sonnleitner; Udo Bläsi

doi:10.3389/fmicb.2018.02488

Titel

Negative Control of RpoS Synthesis by the sRNA ReaL in Pseudomonas aeruginosa

Autor*in

Hue Thi Bach Nguyen

Department für Mikrobiologie, Immunbiologie und Genetik (bis 2023-12-31), Zentrum für Molekulare Biologie (bis 2023-12-31), Universität Wien

David Romero A.

Department für Mikrobiologie, Immunbiologie und Genetik (bis 2023-12-31), Zentrum für Molekulare Biologie (bis 2023-12-31), Universität Wien

Fabian Amman

Institut für Theoretische Chemie, Fakultät für Chemie, Universität Wien

... show all

Abstract

Pseudomonas aeruginosa (Pae) is an opportunistic human pathogen, able to resist host defense mechanisms and antibiotic treatment. In Pae, the master regulator of stress responses RpoS (σS) is involved in the regulation of quorum sensing and several virulence genes. Here, we report that the sRNA ReaL translationally silences rpoS mRNA, which results in a decrease of the RpoS levels. Our studies indicated that ReaL base-pairs with the Shine-Dalgarno region of rpoS mRNA. These studies are underlined by a highly similar transcription profile of a rpoS deletion mutant and a reaL over-expressing strain.

Stichwort

Pseudomonas aeruginosaReaLRpoStranslational silencingsmall RNA

Objekt-Typ

journal article

Sprache

Englisch [eng]

Persistent identifier

phaidra.univie.ac.at/o:933820

DOI

10.3389/fmicb.2018.02488

Erschienen in

Titel