Titel
Translational activation by the noncoding RNA DsrA involves alternative RNase III processing in the rpoS 5'-leader.
Autor*in
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Abstract
The intricate regulation of the Escherichia coli rpoS gene, which encodes the stationary phase sigma-factor sigma(S), includes translational activation by the noncoding RNA DsrA. We observed that the stability of rpoS mRNA, and concomitantly the concentration of sigma(S), were significantly higher in an RNase III-deficient mutant. As no decay intermediates corresponding to the in vitro mapped RNase III cleavage site in the rpoS leader could be detected in vivo, the initial RNase III cleavage appears to be decisive for the observed rapid inactivation of rpoS mRNA. In contrast, we show that base-pairing of DsrA with the rpoS leader creates an alternative RNase III cleavage site within the rpoS/DsrA duplex. This study provides new insights into regulation by small regulatory RNAs in that the molecular function of DsrA not only facilitates ribosome loading on rpoS mRNA, but additionally involves an alternative processing of the target.
Stichwort
DsrAmRNA stabilitynoncoding RNArpoS
Objekt-Typ
Sprache
Englisch [eng]
Persistent identifier
phaidra.univie.ac.at/o:244475
Erschienen in
Titel
RNA
Band
14
Ausgabe
3
Seitenanfang
454
Seitenende
459
Erscheinungsdatum
01.01.2008
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