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Detail o:245127
Titel
Membrane-active compounds activate the transcription factors Pdr1 and Pdr3 connecting pleiotropic drug resistance and membrane lipid homeostasis in Saccharomyces cerevisiae
Autor*in
Christoph
Schüller
Department für Biochemie und Zellbiologie (bis 2023-12-31), Zentrum für Molekulare Biologie (bis 2023-12-31), Universität Wien
Autor*in
Yasmine
Mamnun
Department für Chromosomenbiologie (bis 2023-12-31), Zentrum für Molekulare Biologie (bis 2023-12-31), Universität Wien
Autor*in
Hubert
Wolfger
Max F. Perutz Laboratories
... show all
Abstract
The Saccharomyces cerevisiae zinc cluster transcription factors Pdr1 and Pdr3 mediate general drug resistance to many cytotoxic substances also known as pleiotropic drug resistance (PDR). The regulatory mechanisms that activate Pdr1 and Pdr3 in response to the various xenobiotics are poorly understood. In this study, we report that exposure of yeast cells to 2,4-dichlorophenol (DCP), benzyl alcohol, nonionic detergents, and lysophospholipids causes rapid activation of Pdr1 and Pdr3. Furthermore, Pdr1/Pdr3 target genes encoding the ATP-binding cassette proteins Pdr5 and Pdr15 confer resistance against these compounds. Genome-wide transcript analysis of wild-type and pdr1? pdr3? cells treated with DCP reveals most prominently the activation of the PDR response but also other stress response pathways. Polyoxyethylene-9- laurylether treatment produced a similar profile with regard to activation of Pdr1 and Pdr3, suggesting activation of these by detergents. The Pdr1/Pdr3 response element is sufficient to confer regulation to a reporter gene by these substances in a Pdr1/Pdr3-dependent manner. Our data indicate that compounds with potential membrane-damaging or -perturbing effects might function as an activating signal for Pdr1 and Pdr3, and they suggest a role for their target genes in membrane lipid organization or remodeling. © 2007 by The American Society for Cell Biology.
Objekt-Typ
journal article
Sprache
Englisch
[eng]
Persistent identifier
https://phaidra.univie.ac.at/o:245127
URI
http://www.scopus.com/scopus/inward/record.url?eid=2-s2.0-37049010139&partnerID=40&rel=R7.0.0
DOI
10.1091/mbc.E07-06-0610
Erschienen in
Titel
Molecular Biology of the Cell
Band
18
Ausgabe
12
Seitenanfang
4932
Seitenende
4944
Version
version of record (published version)
Erscheinungsdatum
01.04.2007
Zugänglichkeit
open access
Lizenz
Alle Rechte vorbehalten
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