• Complementary intestinal mucosa and microbiota responses to caloric restriction

    • Kalina Duszka
      Department of Nutritional Sciences, Faculty of Life Sciences, University of Vienna
    • Sandrine Ellero-Simatos
      Toxalim (Research Centre in Food Toxicology), Université de Toulouse, INRA, ENVT, INP-Purpan
    • Ghim Siong Ow
      Bioinformatics Institute, A*STAR Biomedical Sciences Institutes
    • Marianne Defernez
      Quadram Institute Bioscience
    • Eeswari Paramalingam
      Lee Kong Chian School of Medicine, Nanyang Technological University Singapore
    • Adrian Tett
      Quadram Institute Bioscience
    • Shi Ying
      Quadram Institute Bioscience
    • Jürgen König
      Department of Nutritional Sciences, Faculty of Life Sciences, University of Vienna
    • Arjan Narbad
      Quadram Institute Bioscience
    • Vladimir A. Kuznetsov
      Bioinformatics Institute, A*STAR Biomedical Sciences Institutes
    • Hervé Guillou
      Toxalim (Research Centre in Food Toxicology), Université de Toulouse, INRA, ENVT, INP-Purpan
    • Walter Wahli
      Lee Kong Chian School of Medicine, Nanyang Technological University Singapore
  • The intestine is key for nutrient absorption and for interactions between the microbiota and its host. Therefore, the intestinal response to caloric restriction (CR) is thought to be more complex than that of any other organ. Submitting mice to 25% CR during 14 days induced a polarization of duodenum mucosa cell gene expression characterised by upregulation, and downregulation of the metabolic and immune/inflammatory pathways, respectively. The HNF, PPAR, STAT, and IRF families of transcription factors, particularly the Pparα and Isgf3 genes, were identified as potentially critical players in these processes. The impact of CR on metabolic genes in intestinal mucosa was mimicked by inhibition of the mTOR pathway. Furthermore, multiple duodenum and faecal metabolites were altered in CR mice. These changes were dependent on microbiota and their magnitude corresponded to microbial density. Further experiments using mice with depleted gut bacteria and CR-specific microbiota transfer showed that the gene expression polarization observed in the mucosa of CR mice is independent of the microbiota and its metabolites. The holistic interdisciplinary approach that we applied allowed us to characterize various regulatory aspects of the host and microbiota response to CR.

  • PDF

  • http://phaidra.univie.ac.at/o:931163

  • Article

  • Published Version

  • Scientific Reports

  • 2018

  • 8

  • Springer Nature

  • English

  • Open access

  • CC BY Attribution 4.0 International
    © The Author(s) 2018

  • TORNADO – European Union (all programmes)

  • 2045-2322

  • Gastroenterology; Gene expression; Metabolism; Molecular biology